Abstract: A diabetic wound presents a severe risk of infections and other complications because of its
slow healing. Evaluating the pathophysiology during wound healing is imperative for wound care,
necessitating a proper diabetic wound model and assay for monitoring. The adult zebrafish is a rapid
and robust model for studying human cutaneous wound healing because of its fecundity and high
similarities to human wound repair. OCTA as an assay can provide three-dimensional (3D) imaging
of the tissue structure and vasculature in the epidermis, enabling monitoring of the pathophysiologic
alterations in the zebrafish skin wound. We present a longitudinal study for assessing the cutaneous
wound healing of the diabetic adult zebrafish model using OCTA, which is of importance for the
diabetes research using the alternative animal models. We used non-diabetic (n = 9) and type 1
diabetes mellitus (DM) adult zebrafish models (n = 9). The full-thickness wound was generated on
the fish skin, and the wound healing was monitored with OCTA for 15 days. The OCTA results
demonstrated significant differences between diabetic and non-diabetic wound healing, involving
delayed tissue remodeling and impaired angiogenesis for the diabetic wound, leading to slow wound
recovery. The adult zebrafish model and OCTA technique may benefit long-term metabolic disease
studies using zebrafish for drug development.